Every Step Documented, Every Metric Tracked
Click any stage to expand its QC parameters, processing details, and quality benchmarks.
Peripheral Blood Draw & PBMC Isolation
Whole blood collected via standard phlebotomy, PBMCs isolated through density gradient centrifugation within the processing window.
Sample Viability & Cell Quality Assessment
Rigorous viability gating ensures only high-quality, representative samples enter the profiling pipeline.
Maxpar Direct Immune Profiling Assay (MDIPA)
Standardized lyophilized antibody cocktail – 30+ metal-conjugated markers measuring surface and intracellular proteins at single-cell resolution.
Mass Cytometry Data Acquisition
Cells are nebulized, ionized, and analyzed by time-of-flight mass spectrometry – 30+ parameters per cell at thousands of events per second.
Bead Normalization, Gating & QC
Raw FCS files undergo bead normalization, debris removal, and standardized gating before entering the analysis pipeline.
Pennsieve Platform – Structured Data Management
Processed FCS files, annotations, and patient metadata organized in Pennsieve for discoverable, FAIR-compliant data management.
Immune Fingerprinting & Informatics
Comprehensive immune fingerprints – quantifying 50 populations to establish baselines, detect perturbations, and track longitudinal trajectories.